木质素降解菌Bax的筛选及特性研究

为了高效降解造纸污水中木质素类化合物,采用苯胺兰和鞣酸平板法从腐木分离、筛选得到一株具有高降解木质素活性的丝状真菌,经鉴定为绿色木霉,命名为Bax.最适碳源为葡萄糖和蔗糖,最适氮源为蛋白胨和尿紊,最适酸碱度为pH 5.0,最适温度为30℃.通过对木质素氧化酶系分析,主要起作用的是漆酶和木质素酶,为造纸污水的处理奠定了基础.     

抗菌肽及其临床应用研究进展

抗菌肽是生物体在抵抗病原微生物的防御反应过程中产生的一类具有抗微生物活性的小分子多肽。抗菌肽是机体天然免疫系统的重要组成部分,具有广谱的抗革兰氏阳性、阴性菌活性,对真菌、某些有包膜的病毒、寄生虫以及肿瘤细胞也有抑制活性。抗菌肽具有不同于传统抗生素的独特抗菌机制,病原菌不宜对其产生耐药性,有可能成为一种新的抗生素替代品。介绍了抗菌肽的来源与分类、理化特性与生物学活性,并重点阐述其最新的临床应用进展。     

不同音乐环境对小鼠生长发育的影响

目的探讨音乐对小鼠生长发育的影响。方法采用30只ICR小鼠,在50 dB与60 dB的古筝与葫芦丝音乐环境中,饲养27 d,每3天测定体重一次,每9天测定饲料各种营养消化率一次。结果雌鼠体重在不同音乐环境中都有显著提高（P〈0.05）,且50 dB组高于60 dB组（P〈0.05）。雄鼠体重在古筝音乐环境中有显著提高（P〈0.05）;而在葫芦丝音乐环境中有显著降低。在不同音乐环境中,鼠的各种营养物质消化率无显著差异（P〉0.05）。结论不同的音乐环境对小鼠的体重有显著影响,而对小鼠的各种营养物质的消化率无显著影响。     

Economically and ecologically important plant communities in high altitude coniferous forest of Malam Jabba,Swat, Pakistan

A study on the economically important plant communities was carried out during summer 2008 in various parts of Malam Jabba valley, Swat. The principal aim of the study was phytosociological evaluation with special reference to the occurrence of commercially important medicinal plant species in coniferous forest of the study area. Secondly to prepare ethnobotanical inventory of the plant resources of the area, as well as to evaluate the conservation status of important medicinal and aromatic plants (MAPs) through rapid vulnerable assessment (RVA) procedure. The study documented 90 species of ethnobotanical importance, out of these 71 spp used as medicinal plant, 20 spp fodder plant, 10 spp vegetables, 14 spp wild fruit, 18 spp fuel wood, 9 spp furniture and agricultural tools, 9 spp thatching, fencing and hedges, 4 spp honey bee, 2 spp evil eyes, 2 spp religious and 3 spp as poison. Phytosociologically six plant communities were found, comprising five herbs-shrubs-trees communities and one meadow community. Further study is, therefore, required to quantify the availability of species and to suggest suitable method for their production and conservation. Recommendations are also given in the spheres of training in identification, sustainable collection, value addition, trade monitoring and cooperative system of marketing.     

Difference of pine ectomycorrhizal biomass in relation to forest conditions

In this study, two plots in a secondary and another two in planted Pinus densiflora stands were exposed to different forest treatments, and the ectomycorrhizal (EM) biomass and its ergosterol content was measured for a year. The unmanaged plot in the secondary stand had greater EM biomass than those in any other plots. Whereas understory cutting had less effect on EM biomass, litter and humus removal decreased pine EM biomass and its ergosterol content, suggesting that such forest treatment alters EM biomass and its structure.     

血S100B蛋白和尿乳酸/肌酐比值对门静脉高压症术后肝性脑病发生的早期预测价值

目的评价血S100B蛋白和尿乳酸/肌酐对乙肝肝硬化门脉高压症术后肝性脑病发生的早期预测价值。方法回顾性分析65例乙肝肝硬化门脉高压症患者的临床资料,动态检测术后24、48和72h的血S100B蛋白和尿乳酸/肌酐比值水平,根据是否发生术后肝性脑病将受试者分为肝性脑病组与非肝性脑病组,并对肝性脑病组患者进行临床分度。结果乙肝肝硬化门脉高压症患者术后发生肝性脑病组72h内血S100B蛋白含量和24 h内尿乳酸/肌酐比值水平明显高于非肝性脑病组(P<0.001);72h内血S100B蛋白含量和24 h内尿乳酸/肌酐比值之间及与肝性脑病的临床分度呈正相关(P<0.001);当血S100B水平在28ng/L,尿乳酸/肌酐比值在0.47时,单独检测72h血S100B蛋白的敏感度、特异度分别为91.2%、93.6%;尿乳酸/肌酐比值预测肝性脑病的敏感度和特异性度以术后24h最高,分别为89.3%和91.7%;如检测72h血S100B蛋白的同时监测术后24h尿乳酸/肌酐比值可显著提高肝性脑病诊断的准确性,联合应用两项指标进行检测,诊断的敏感度和特异度分别为95.7%和98.6%,较两种方法单独应用敏感度和特异度均提高。结论对门静脉高压症患者术后以临床表现为基础,同时监测72h内血S100B蛋白和24h尿乳酸/肌酐比值,对提高术后肝性脑病的早期诊断和临床分度具有一定应用价值。     

植物bHLH转录因子的结构特点及其生物学功能

bHLH(basic/helix-loop-helix)转录因子在真核生物的生长发育过程中起着重要的调控作用,它们组成了转录因子中的一个大家族。bHLH转录因子不仅普遍参与了植物的生长发育,包括毛状体的发生、光形态建成和光信号转导,而且在植物响应逆境胁迫和次生代谢方面也具有重要作用。对植物bHLH转录因子的结构特点及其生物学功能进行综述。     

肉制品腐败变质原因分析

对真空软包装肉制品腐败变质原因分析表明：生物性因素是主要原因之一,污染微生物是乳酸球菌和芽胞杆菌;变质产品中的微生物数量及因此造成的酸化,不足以单方面造成产品变质,与产品含水量有关,是生物因素与非生物因素联合作用导致产品液化胀袋而变质;乳酸球菌由污染的冻结肉携带,而污染的芽胞杆菌是生产器具消毒不彻底造成的。     

湖北西部山地革螨及恙螨名录

自1989年以来,先后从湖北西部山地6个不同海拔、12个不同类型生境捕获的啮齿、食虫等小型哺乳动物体外采集到一大批革螨、恙螨和吸虱标本,经初步整理,鉴定有革螨l1科32属73种、恙螨有3亚科11属41种和亚种、吸虱3种,其中有中国新纪录1种,籁氏巨刺螨Macronyssus leucippe（Domrow）和湖北新纪录41种。     

大豆根瘤菌HH103菌株培养基的筛选与优化

以快生型大豆根瘤菌HH103菌株为供试菌株,采用单因素碳氮源利用试验和正交设计试验,确定最佳培养基及其配方。结果表明：该菌株在YMA中生长良好,最佳碳源为蔗糖,最佳氮源为酵母膏,最佳培养基成分配方（g/L）：蔗糖11,酵母膏0.9,K2HPO4 0.5,MnSO4 0.005,CaCl2 0.1,KH2PO4 0.5,MgSO4 0.2,KNO30.77,（NH4）2HPO4 0.33,FeCl3 0.005,pH 7.2。